Golestan University of Medical Sciences Repository

Increased expression of miR-146a, miR-10b, and miR-21 in cancer stem-like gastro-spheres

Bakhshi, M. and Asadi, J. and Ebrahimi, M. and Moradi, A.-V. and Hajimoradi, M. (2019) Increased expression of miR-146a, miR-10b, and miR-21 in cancer stem-like gastro-spheres. Journal of Cellular Biochemistry, 120 (10). pp. 16589-16599.

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Abstract

Background: Gastric cancer remains one of the leading causes of cancer-associated mortalities globally. Accumulating evidence support the presence of gastric cancer stem cells (CSCs) and their role in the pathogenesis and therapeutic challenges of gastric cancer. MicroRNAs (miRNAs) may be influenced by the cellular differentiative state and as critical regulators of the cellular fate in development and cancer, can modulate the behavior of CSCs too. Here, we aimed to investigate the expression relevance of three prognostic miRNAs (miR-21, miR-10b, and miR-146a) in CSCs of AGS and MKN-45 gastric cancer cell lines. Methods: Serial sphere-forming assay in serum-free culture medium was used to enrich the cellular population with stem-like properties. Gastro-spheres were characterized by evaluating the stemness gene expression, clonogenicity, and resistance to docetaxel and cisplatin in comparison with their parental cells. The expression level of miRNAs in gastro-spheres and their parental cells was measured using quantitative reverse transcription polymerase chain reaction. Results: Gastro-spheres from both cell lines exhibit stem-like properties: upregulated stemness associated genes (P < 0.05), more colonogenicity and more resistance to docetaxel (P < 0.05). MKN-45 gastro-spheres exhibited upregulated expression of miR-21 (1.8-folds), miR-10b (1.34-folds) and miR-146a (4.8-folds; P < 0.05) compared with the parental cells. AGS-derived gastro-spheres showed upregulation of miR-21 (4.7-folds; P < 0.01), miR-10b (15.2-folds; P < 0.001) and miR-146a (39.3-folds; P < 0.05). Conclusion: Our data exhibited upregulation of miR-21, miR-10b, and miR-146a in the stem-like gastro-spheres; however; their function in gastric CSCs remains to be verified by further experiments. © 2019 Wiley Periodicals, Inc.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: cisplatin; docetaxel; messenger RNA; microRNA 10b; microRNA 146a; microRNA 21, AGS cell line; Article; cancer prognosis; cancer resistance; cancer stem cell; cell self-renewal; clonogenesis; controlled study; gene expression regulation; gene overexpression; MKN45 cell line; priority journal; quantitative analysis; reverse transcription polymerase chain reaction; stomach cancer; upregulation
Subjects: سیستم گوارشی WI
QS آناتومی انسان
آسیب شناسی QZ
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 21 Dec 2019 06:02
Last Modified: 21 Dec 2019 06:02
URI: http://eprints.goums.ac.ir/id/eprint/10452

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