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Molecular detection of Campylobacter jejuni in patients with Crohn's disease in Iran

Asiabar, A.S. and Aghdaei, H.A. and Zamani, S. and Bokaie, S. and Zali, M.R. and Feizabadi, M.M. (2019) Molecular detection of Campylobacter jejuni in patients with Crohn's disease in Iran. Medical Journal of the Islamic Republic of Iran, 33 (1).

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Abstract

Background: Crohn's disease is one of the most significant intestinal disorders and is known as inflammatory bowel disease; Campylobacter spp. are one of the leading causes of bacterial gastroenteritis in humans. Methods: In this study, 60 tissue samples, including 30 cases with Crohn's disease and 30 cases with no inflammatory bowel disease, were collected. Patients were referred to Taleghani hospital and Behboud clinic between March 2015 and May 2016. Biopsies were used for DNA extraction and assessment of Campylobacter jejuni in patients with Crohn's disease and controls using polymerase chain reaction and quantitative real-time polymerase chain reaction. All positive amplified fragments were sequenced. The gene encoding 16S rRNA, specific to Campylobacter genus, was amplified. Results: The results were positive for Campylobacter genus in patients with Crohn's disease compared to healthy individuals. The quantitative real-time PCR showed a significantly higher prevalence of Campylobacter jejuni, particularly in hippurate hydrolase in tissue specimens of patients with Crohn's disease compared to control group. The correlation between Campylobacter jejuni and diarrhea symptoms in patients with Crohn's disease and controls was investigated. One positive case of Campylobacter jejuni found in patients without diarrhea was compared with 13 patients with diarrhea. Conclusion: The present study demonstrated the alarmingly high rate of Campylobacter jejuni prevalence in Crohn's disease patients with diarrhea symptoms. However, further investigation is needed to determine the possible causing factors of this disease. © Iran University of Medical Sciences.

Item Type: Article
Additional Information: cited By 0
Subjects: سیستم گوارشی WI
QS آناتومی انسان
QTفیزیولوژی
QU بیوشیمی
آسیب شناسی QZ
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 16 Dec 2019 09:59
Last Modified: 16 Dec 2019 09:59
URI: http://eprints.goums.ac.ir/id/eprint/10445

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