Golestan University of Medical Sciences Repository

Altered Micro-RNA Regulation and Neuroprotection Activity of Eremostachys labiosiformis in Alzheimer's Disease Model

Samandari-Bahraseman, M.R. and Jahanshahi, M. and Asadi Barbariha, S. and Elyasi, L. (2019) Altered Micro-RNA Regulation and Neuroprotection Activity of Eremostachys labiosiformis in Alzheimer's Disease Model. Annals of Neurosciences, 25 (3). pp. 160-165.

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Background: Amyloid-β peptide (Aβ) is involved in the formation of senile plaques in Alzheimer's disease (AD), and causes neuronal cell death by inducing oxidative stress. Objective: We investigated the protective effect of Eremostachys labiosiformis extract against the Aβ-induced toxicity in SH-SY5Y cells. Methods: Methanolic extract from the aerial parts of E. labiosiformis was prepared by percolation method at room temperature. SH-SY5Y cells were treated and incubated with different concentrations of the extract for 1 h, before addition of Aβ. Cytotoxicity was measured 24 h after the addition of Aβ to the medium using MTT and reactive oxygen species (ROS) assays. Effective doses were evaluated using real-time polymerase chain reaction to evaluate expression of miR-212 and miR-132. The results were analyzed using SPSS software (16). Results: Exposure of -SH-SY5Y cells to Aβ significantly affected the viability of cells and increased ROS levels. The results revealed that 1.2 and 2.5 μg/mL of the E. labiosiformis extract reduced Aβ-induced deterioration. Only 2.5 μg/mL of the extract could reduce ROS levels. In addition, 5 μg/mL of the extract increased the expression of the miRNAs, which was reduced after exposure to Aβ. Conclusion: Based on the antioxidant and protective effects of the E. labiosiformis extract on expression of miR-132 and miR-212 and ROS level, this herb could be used as a suitable candidate for future studies on neurodegenerative diseases including AD. © 2018 S. Karger AG, Basel. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Eremostachys labiosiformis extract; microRNA; microRNA 132; microRNA 212; neuroprotective agent; plant extract; reactive oxygen metabolite; unclassified drug, Alzheimer disease; Article; cell death; cell viability; controlled study; Eremostachys labiosiformis; intracellular space; Lamiaceae; medicinal plant; mortality rate; neuroprotection; real time polymerase chain reaction; SH-SY5Y cell line
Subjects: سیستم عصبی WL
QS آناتومی انسان
QU بیوشیمی
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 16 Dec 2019 09:56
Last Modified: 16 Dec 2019 09:56
URI: http://eprints.goums.ac.ir/id/eprint/10444

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