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Evaluation of IL-17 serum level, brain inflammation and demyelination in experimental autoimmune encephalomyelitis C57BL/6 mice model with different doses of myelin oligodendrocyte glycoprotein

Ghorbani, M.M. and Farazmandfar, T. and Nasirikenari, M. and Abediankenari, S. and Meamarian, A. and Shahbazi, M. (2019) Evaluation of IL-17 serum level, brain inflammation and demyelination in experimental autoimmune encephalomyelitis C57BL/6 mice model with different doses of myelin oligodendrocyte glycoprotein. Iranian Journal of Allergy, Asthma and Immunology, 18 (3). pp. 300-309.

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Abstract

Multiple sclerosis (MS) is an autoimmune disease that affects the central nervous system.MS creates a wide range of symptoms with lifelong debilitating consequences. The hallmark of the disease is the inflammation of the nervous system, which can lead to damage to the nerve tissue and loss of function of the neurons. IL-17 has a prominent role in the beginning of inflammatory reactions. Here, we analyzed a mouse model developed using anti-myelin antibodies. This mouse model mimics many symptoms of MS in humans. C57BL/6 mice were randomly divided into five groups. Mice were immunized subcutaneously with 50 μg, 100 μg, 150 μg and 200 μg myelin oligodendrocyte glycoprotein in complete Freund's adjuvant containing 4 mg/Ml Mycobacterium tuberculosis and two injections of 800 ng of pertussis toxin intraperitoneally, on day 0 and 2 post immunization. Serum level of IL-17 was measured, inflammation and demyelination of brain tissue were also evaluated. Mice with experimental autoimmune encephalomyelitis demonstrated inflammatory cell accumulation, different degrees of demyelination in the brain, and rising levels of serum IL-17 depending on the dose of the anti-myelin antibody. Our study demonstrates that level of IL-17 production is directly associated with inflammation and demyelination. In addition, different degrees of experimental autoimmune encephalomyelitis in mice can be utilized to test a wide range of therapeutic interventions for MS treatment. Copyright © June 2019, Iran J Allergy Asthma Immunol. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Freund adjuvant; interleukin 17; myelin oligodendrocyte glycoprotein; pertussis toxin, animal experiment; animal model; animal tissue; Article; blood level; body weight loss; brain tissue; C57BL 6 mouse; cell infiltration; controlled study; demyelinating disease; disease severity; encephalitis; experimental autoimmune encephalomyelitis; female; immunization; inflammatory cell; mouse; multiple sclerosis; Mycobacterium tuberculosis; nonhuman
Subjects: سیستم عصبی WL
بیماریهای تغذیه ومتابولیکی WD
QS آناتومی انسان
QTفیزیولوژی
QU بیوشیمی
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 16 Dec 2019 11:28
Last Modified: 16 Dec 2019 11:28
URI: http://eprints.goums.ac.ir/id/eprint/10436

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