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Induction of cross-tolerance between protective effect of morphine and nicotine in 6-hydroxydopamine-induce neurotoxicity in SH-SY5Y human dopaminergic neuroblastoma cells

Elyasi, L. and Eftekhar-Vaghefi, S.H. and Asadi-Shekaari, M. and Esmaeili-Mahani, S. (2019) Induction of cross-tolerance between protective effect of morphine and nicotine in 6-hydroxydopamine-induce neurotoxicity in SH-SY5Y human dopaminergic neuroblastoma cells. International Journal of Neuroscience, 129 (2). pp. 129-138.

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Purpose: Parkinson�s disease is a progressive neurodegenerative disease characterized by progressive and selective death of dopaminergic neurons. It has been reported that nicotine and morphine have protective roles during neuronal damage in Parkinson�s disease. In addition, the induction of cross-tolerance between their biological effects has been shown in numerous reports. Methods: Here, we investigated the effects of nicotine and morphine on 6-OHDA-induced neurotoxicity in human neuroblastoma SH-SY5Y cell line as an in vitro model of Parkinson�s disease. Cell damage was induced by 150 μM 6-OHDA and the cells viability was examined by MTT assay. Intracellular reactive oxygen species, calcium level, and mitochondrial membrane potential were determined by fluorescence spectrophotometer method. Biochemical markers of apoptosis were also evaluated by immunoblotting. Result: The data showed that morphine and nicotine prevent 6-OHDA- induced cell damage and apoptosis. However, the protective effects of nicotine were not observed in chronic morphine-pretreated cells. Morphine had no protective effects in chronic nicotine-incubated cells. Conclusion: A cross-tolerance between protective effects of morphine and nicotine was occurred in 6-OHDA-induced SH-SY5Y cell toxicity. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: biochemical marker; bungarotoxin receptor; calcium; caspase 3; cytochrome c; messenger RNA; morphine; mu opiate receptor; nicotine; oxidopamine; reactive oxygen metabolite; morphine; neuroprotective agent; nicotine; reactive oxygen metabolite, apoptosis; Article; calcium cell level; cell damage; cell viability; concentration response; controlled study; dopaminergic nerve cell; drug cross tolerance; drug effect; human; human cell; in vitro study; mitochondrial membrane potential; mRNA expression level; neuroprotection; neurotoxicity; Parkinson disease; receptor down regulation; SH-SY5Y cell line; spectrofluorometry; cell survival; chemically induced; DNA fragmentation; dopaminergic nerve cell; drug tolerance; metabolism; Parkinson disease; parkinsonism; tumor cell line, Apoptosis; Cell Line, Tumor; Cell Survival; DNA Fragmentation; Dopaminergic Neurons; Drug Tolerance; Humans; Morphine; Neuroprotective Agents; Nicotine; Oxidopamine; Parkinson Disease; Parkinsonian Disorders; Reactive Oxygen Species
Subjects: سیستم عصبی WL
فارماکولوژی QV > شیمی دارویی
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مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 15 Dec 2019 12:04
Last Modified: 15 Dec 2019 12:04
URI: http://eprints.goums.ac.ir/id/eprint/10390

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