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Reproducibility of 15-Loci MIRU-VNTR method in mycobacterium tuberculosis genotyping

Kochkaksaraei, M.B. and Kaboosi, H. and Ghaemi, E.A. (2019) Reproducibility of 15-Loci MIRU-VNTR method in mycobacterium tuberculosis genotyping. Jundishapur Journal of Microbiology, 12 (8).

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Abstract

Background: Mycobacterium tuberculosis genotyping is an essential step for understanding the epidemiology of tuberculosis. Mycobacterial-interspersed-repetitive-units (MIRU)-variable-number-of-tandem-repeats (VNTR) typing is an important method for this purpose. Objectives: The study aimed to determine the reproducibility of 15-loci MIRU-VNTR method. Methods: DNA extraction and 15-loci MIRU-VNTR were carried out for genotyping of 60 M. tuberculosis isolates collected from different clinical samples of 27 M. tuberculosis patients, each with two to four clinical isolates of M. tuberculosis. The similarity of M. tuberculosis isolates of the same patient was investigated by MIRU-VNTRplus. Results: The patterns of MIRU-VNTR were identical in 43 M. tuberculosis isolates collected from 20 tuberculosis patients, giving the repeatability of 71.6 for the test. In 12 isolates of M. tuberculosis belonging to five patients, the variable-number tandem repeat (VNTR) in only one locus was different. In three M. tuberculosis isolates of one patient, two different genotypes were identified. Conclusions: This study confirms the high reproducibility of 15-loci MIRU-VNTR, except for limited cases. © 2019, Author(s).

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Article; bacterium isolate; clinical article; controlled study; DNA extraction; gene dosage; gene locus; genetic variability; genotype; genotyping technique; human; interspersed repeat; Mycobacterium tuberculosis; nonhuman; polymerase chain reaction; reproducibility; tuberculosis; variable number of tandem repeat
Subjects: بیماریهای واگیرWC
میکروب شناسی وایمنی شناسی QW
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 10 Dec 2019 07:08
Last Modified: 10 Dec 2019 07:08
URI: http://eprints.goums.ac.ir/id/eprint/10367

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