Golestan University of Medical Sciences Repository

Amino acid mutation in Plasmodium vivax dihydrofolate reductase (dhfr) and dihydropteroate synthetase (dhps) genes in Hormozgan Province, southern Iran

Maghsoodloorad, S. and Hosseinzadeh, N. and Haghighi, A. and Solgi, R. and Yusuf, M. and Hatam, G. (2019) Amino acid mutation in Plasmodium vivax dihydrofolate reductase (dhfr) and dihydropteroate synthetase (dhps) genes in Hormozgan Province, southern Iran. Journal of Vector Borne Diseases, 56 (2). pp. 170-173.

Full text not available from this repository.

Abstract

Molecular analysis of antifolate resistance-associated genes - dihydrofolate reductase (dhfr) and dihydropteroate synthetase (dhps) of Plasmodium vivax is important in predicting the emergence of drug resistance to sulphadoxine-pyrimethamine (SP). The present study aimed to determine the polymorphism of dhfr and dhps genes in P. vivax field isolates. Samples from 80 microscopically diagnosed vivax malaria cases were collected from endemic areas of malaria in Hormozgan Province of Iran, from June 2010 to November 2015. The two sets of codons at position 33, 57, 58, 117, 173 of dhfr and 382, 383, and 553 of dhps genes were analysed by direct sequencing of PCR products. The majority of the isolates (70) harboured a wild-type allele for P. vivax dhfr (Pvdhfr) and P. vivax dhps (Pvdhps). Mutations were detected in three codons of Pvdhfr (P33L, S58R and S117N) and single codon in Pvdhps (A383G). Novel mutations that have not been identified previously at codon 459 (D459A) of Pvdhps were also observed. The high prevalence of point mutation as well as the rising triple mutation of Pvdhfr and Pvdhps genotypes necessitate change in programmes and guidelines to eliminate P. vivax in future. © 2019 Journal of Vector Borne Diseases. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Subjects: بیماریهای واگیرWC
آسیب شناسی QZ
انگل شناسی QX
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 22 Sep 2019 11:37
Last Modified: 22 Sep 2019 11:37
URI: http://eprints.goums.ac.ir/id/eprint/10334

Actions (login required)

View Item View Item