Golestan University of Medical Sciences Repository

Molecular and serologic characterization of rotavirus from children with acute gastroenteritis in northern Iran, Gorgan

Lorestani, N. and Moradi, A. and Teimoori, A. and Masodi, M. and Khanizadeh, S. and Hassanpour, M. and Javid, N. and Ardebili, A. and Tabarraei, A. and Nikoo, H.R. (2019) Molecular and serologic characterization of rotavirus from children with acute gastroenteritis in northern Iran, Gorgan. BMC Gastroenterology, 19 (1).

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Background: The pattern and distribution of human rotavirus genotypes in young children in developing countries play an important role in epidemiological studies, as well as providing a strategy for the development of future rotavirus vaccine. Methods: We evaluated stool samples from 349 children with acute gastroenteritis from Northern Iran (Gorgan city, Golestan province). Polyacrylamide Gel Electrophoresis (PAGE) and Latex Agglutination Test (LAT) were utilized to determine the prevalence of human rotavirus in fecal samples. Moreover semi-multiplex RT-PCR technique was carried out in order to determine the P and G genotypes of human rotavirus in rotavirus-positive samples. Results: A total of 46 rotavirus-positive samples were G and P genotyped. Whereas 28 (60.8) fecal specimens contained only one rotavirus strain, 14 (30.4) were mixed rotavirus infections and 4 (8.8) was non-typeable. Overall, during the study, 57.82 of strains identified as genotype G1, G2 (18.70), G3 (4.69), G4 (3.13), G8 (3.13), G9 (6.26) and non-typeable G (6.26). From all these mentioned rotavirus strains, 46 were characterized as P 8 (97.80%) and P 4 (2.20%).Our analysis of the G and P genotyping of strains from all 46 rotavirus-infected children has revealed that 4/46(6.26%) of G type strains were non-typeable. The predominant single G/P combination was G1P 8 (57.82%), followed by, G2P 8 (16.98%), G2P 4 (1.72%), G3P 8 (4.69%), G4P 8 (3.13%) G8P 8 (3.13%), G9P 8 (6.26%) and four cases of non-typeable G (6.26%). Rotavirus was detected in 39 specimens (11.17%) by PAGE and in 38 specimens (10.88%) by LAT. Both tests were 100% specific; however, the LAT was 82.61% sensitive compared to the PAGE, which was 84.78% sensitive. Conclusions: The results suggest that to characterize rotavirus strains as well as design new effective vaccines for children with acute gastroenteritis, a large-scale study is needed in future. © 2019 The Author(s).

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Rotavirus vaccine, acute gastroenteritis; Article; child; developing country; diagnostic test accuracy study; drug effect; feces analysis; female; genotype; human; infant; Iran; latex agglutination test; major clinical study; male; molecular interaction; multiplex polymerase chain reaction; pathogenesis; pediatrics; polyacrylamide gel electrophoresis; preschool child; prevalence; reverse transcription polymerase chain reaction; Rotavirus; Rotavirus infection; sensitivity and specificity; serology; serotyping; vaccination; virus detection; virus strain
Subjects: میکروب شناسی وایمنی شناسی QW
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 22 Sep 2019 10:22
Last Modified: 22 Sep 2019 10:22
URI: http://eprints.goums.ac.ir/id/eprint/10308

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