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Inhibition of sirtuin 1 deacetylase by miR-211-5p provides a mechanism for the induction of cell death in breast cancer cells

Yarahmadi, S. and Abdolvahabi, Z. and Hesari, Z. and Tavakoli-Yaraki, M. and Yousefi, Z. and Seiri, P. and Hosseinkhani, S. and Nourbakhsh, M. (2019) Inhibition of sirtuin 1 deacetylase by miR-211-5p provides a mechanism for the induction of cell death in breast cancer cells. Gene, 711.

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Abstract

Sirtuin 1 is one of the regulators of cell growth and survival and its inhibition is suggested as a suitable mechanism to overcome breast cancer development. In this study we explored the role of miR-211-5p in SIRT1/p53 pathway and its influence on breast cancer cell viability and apoptosis. Cells were transfected with miR-211-5p mimic and inhibitor to modulate cellular miR-211-5p levels in breast cancer cell lines, MDA-MB-231 and MCF-7. Gene expression of miR-211-5p and SIRT1 were measured with real-time PCR. SIRT1 protein level and the acetylation of p53 as well as SIRT1 activity were evaluated by Western blotting and fluorometry, respectively. In order to explore the direct attachment of miR-211-5p to the 3�-UTR of SIRT1 mRNA, luciferase reporter assay was applied. Cell viability in response to miR-211-5p was studied by MTT assay and apoptosis was assessed by annexin V labeling followed by flow cytometry. Results showed that SIRT1 gene and protein expression were inhibited by miR-211-5p and the 3�-UTR of SIRT1 was found to be directly targeted by miR-211-5p. Inhibition of SIRT1 expression resulted in its reduced activity. Up-regulation of miR-211-5p was also followed by a significant decline in the acetylation status of p53 which was associated with remarkable decreased cell viability and induction of apoptosis in breast cancer cells. Antisense oligonucleotide of miR-211-5p acted as its inhibitor and exerted opposite effects both on SIRT1 expression and cell apoptosis. In conclusion, inhibition of SIRT1 by miR-211-5p could effectively reduce breast cancer cell survival and cause cell death and therefore might be considered a seemly mechanism for designing anticancer therapies. © 2019 Elsevier B.V.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: microRNA; MIRN211 microRNA, human; protein p53; SIRT1 protein, human; sirtuin 1; TP53 protein, human, 3' untranslated region; acetylation; breast tumor; cell survival; female; gene expression regulation; genetics; human; MCF-7 cell line; metabolism; tumor cell line, 3' Untranslated Regions; Acetylation; Breast Neoplasms; Cell Line, Tumor; Cell Survival; Female; Gene Expression Regulation, Neoplastic; Humans; MCF-7 Cells; MicroRNAs; Sirtuin 1; Tumor Suppressor Protein p53
Subjects: بیماریهای زنان WP
فارماکولوژی QV
> دانشکده داروسازی > فارماکولوژی QV

آسیب شناسی QZ
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 22 Sep 2019 08:15
Last Modified: 22 Sep 2019 08:15
URI: http://eprints.goums.ac.ir/id/eprint/10288

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