Golestan University of Medical Sciences Repository

Molecular typing of Clostridioides difficile isolates from clinical and non-clinical samples in Iran

Kouhsari, E. and Douraghi, M. and Fakhre Yaseri, H. and Talebi, M. and Ahmadi, A. and Sholeh, M. and Amirmozafari, N. (2019) Molecular typing of Clostridioides difficile isolates from clinical and non-clinical samples in Iran. APMIS, 127 (4). pp. 222-227.

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Clostridioides difficile is a major cause of nosocomial infectious diarrhea in hospitalized patients throughout the world. We aimed to characterize C. difficile isolates among hospitalized patients, hospital staffs, and hospital environment samples obtained in three tertiary care hospitals of Iran with regard to their molecular types between June 2016 and November 2017. The toxigenicity of C. difficile isolates was determined by toxigenic culture and multiplex-PCR. Toxigenic C. difficile isolates collected were ribotyped using capillary gel electrophoresis-based PCR and the database of WEBRIBO (http://webribo.ages.at). Of 500 clinical and non-clinical samples, toxigenic C. difficile were identified in 35 of 250 stool samples (14) and in 3 of 250 swabs (1.2). The most frequently found ribotypes (RTs) were 039, AI-12, and AI-21 (15.8, 10.52, and 10.52 of all isolates, respectively). Further RTs were: 017, 001, AI-3, AI-15, AI-18, AI-10, AI-4, and PR21195 (as new ribotype). The epidemic RTs (027 and 078) seen in the Europe, North America, and Asia were completely absent in this study. © 2019 APMIS. Published by John Wiley & Sons Ltd

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: adult; aged; Article; Asia; bacterium isolate; bacterium isolation; Clostridium difficile infection; controlled study; data base; Europe; feces analysis; female; gel electrophoresis; hospital patient; hospital personnel; human; Iran; major clinical study; male; middle aged; molecular typing; multiplex polymerase chain reaction; North America; Peptoclostridium difficile; polymerase chain reaction; priority journal; ribotyping; tertiary care center; very elderly; capillary electrophoresis; classification; Clostridium infection; cross infection; diarrhea; environmental microbiology; genetics; isolation and purification; microbiology; multiplex polymerase chain reaction; Peptoclostridium difficile; ribotyping, bacterial toxin, Aged; Aged, 80 and over; Bacterial Toxins; Clostridium difficile; Clostridium Infections; Cross Infection; Diarrhea; Electrophoresis, Capillary; Environmental Microbiology; Epidemiologic Studies; Female; Humans; Iran; Male; Middle Aged; Multiplex Polymerase Chain Reaction; Ribotyping; Tertiary Care Centers
Subjects: بیماریهای واگیرWC
میکروب شناسی وایمنی شناسی QW
بهداشت عمومی WA
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 01 Jul 2019 08:17
Last Modified: 01 Jul 2019 08:17
URI: http://eprints.goums.ac.ir/id/eprint/10228

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