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Antimicrobial resistance, prevalence of resistance genes, and molecular characterization in intestinal Bacteroides fragilis group isolates

Kouhsari, E. and Mohammadzadeh, N. and Kashanizadeh, M.G. and Saghafi, M.M. and Hallajzadeh, M. and Fattahi, A. and Ahmadi, A. and Niknejad, F. and Ghafouri, Z. and Asadi, A. and Boujary Nasrabadi, M.R. (2019) Antimicrobial resistance, prevalence of resistance genes, and molecular characterization in intestinal Bacteroides fragilis group isolates. APMIS, 127 (6). pp. 454-461.

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Abstract

Since the level of antimicrobial resistance in Bacteroides fragilis has increased, monitoring the antimicrobial susceptibility could be necessary. The objectives of this study were to (i) investigate the prevalence of species, the occurrence of reduced antimicrobial susceptibility (E-test method), and antibiotic resistance genes in the B. fragilis group and (ii) evaluate the prevalence of enterotoxigenic B. fragilis and the distribution of bft gene subtypes in hospitalized patients. As many as 475 isolates out of 250 stool samples were detected to be B. fragilis group by using conventional biochemical tests (API-32A system) and multiplex-PCR. In addition, 48.2, 13.9, 76.6, and 1.2 of B. fragilis group isolates were resistant (according to EUCAST breakpoint) to piperacillin-tazobactam, meropenem, clindamycin, and metronidazole, respectively. Six metronidazole-resistant strains were isolated; B. fragilis (n: 3), B. thetaiotaomicron, B. vulgates, and B. ovatus. The presence of the cfiA, cepA, ermF, and nim genes was observed in 3.8, 15.9, 34.1, and 0.7 of the B. fragilis isolates, respectively. One hundred thirty-two B. fragilis isolates (27.8)and 21 B fragilis isolates (15.9) turned out to be bft gene positive by multiplex-PCR; eleven isolates (52.4) harbored bft-1, eight isolates (38) harbored bft-2 isotypes, and two isolates (9.5) harbored bft-3 isotype (16.66). These bacteria harbor antimicrobial resistance genes that could be transferred to other susceptible intestinal strains. Further investigations on lineage analysis are needed for a better understanding of these bacteria in Iran. © 2019 APMIS. Published by John Wiley & Sons Ltd

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: adolescent; adult; aged; antibiotic resistance; Bacteroides fragilis; child; drug effect; feces; female; genetics; human; intestine; male; microbiology; middle aged; prevalence; very elderly; young adult, Adolescent; Adult; Aged; Aged, 80 and over; Bacteroides fragilis; Child; Drug Resistance, Bacterial; Feces; Female; Humans; Intestines; Male; Middle Aged; Prevalence; Young Adult
Subjects: سیستم گوارشی WI
بیماریهای واگیرWC
فارماکولوژی QV
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میکروب شناسی وایمنی شناسی QW
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 01 Jul 2019 07:25
Last Modified: 01 Jul 2019 07:25
URI: http://eprints.goums.ac.ir/id/eprint/10215

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