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Immunization of mice by the co-administration of codon-optimized HPV16 E7 and lL12 genes against HPV16-associated cervical cancer

Ajorloo, M. and Alamdary, A. and Soleimanjahi, H. and El Boulani, A. and Khanizadeh, S. and Nikoo, H.R. (2019) Immunization of mice by the co-administration of codon-optimized HPV16 E7 and lL12 genes against HPV16-associated cervical cancer. Microbial Pathogenesis, 132. pp. 20-25.

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Abstract

Background: Various promising procedures have been used to improve the potency of DNA vaccines for the treatment of human papillomavirus type 16 (HPV16) infections. Interleukin-12 (IL12) is a powerful adjuvant that can contribute to T cell-mediated protection against many pathogens, specifically viruses. Considering the important role of T cell-mediated immunity in tumor clearance, the induction of these responses can help control the progression of tumors in animal models. We have demonstrated that the co-administration of codon-optimized E7 (uE7) gene of HPV16 with interleukin-12 is effective in the development of antitumor responses. Objectives: The present study examined the co-administration of codon-optimized HPV16 E7 gene with murine interleukin-12 gene (mIL-12) as a vaccine adjuvant in tumor mice model. Materials and methods: C57BL/6 mice were studied for tumor progression after injection of recombinant DNA vaccines. Lactate dehydrogenase (LDH) and IFN-γ were measured to evaluate the activity of cytotoxic T lymphocytes (CTLs). Measurements of tumor volume and MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay were used for assessment of therapeutic antitumor effects of the vaccines. Results: Results showed that DNA vaccines, specifically codon-optimized E7/murine interleukin-12 (mIL-12), elicited significant differences in levels of IFN-γ and cytotoxic T lymphocyte (CTLs) responses compared to control groups. Furthermore, higher antitumor response and lower tumor size in the vaccine group was significantly evident compared to control group. Conclusion: The co-administration of codon-optimized HPV16 E7 gene with IL12 significantly enhances the DNA vaccine potency against HPV16-associated cervical cancer. © 2019 Elsevier Ltd

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: DNA vaccine; gamma interferon; interleukin 12; interleukin 4; lactate dehydrogenase; protein E7; recombinant vaccine, animal cell; animal experiment; animal model; animal tissue; antineoplastic activity; Article; C57BL 6 mouse; cancer immunization; codon; controlled study; cytokine production; cytotoxic T lymphocyte; drug effect; drug efficacy; drug response; female; Human papillomavirus type 16; lymphocyte proliferation; mouse; MTT assay; nonhuman; priority journal; tumor growth; tumor volume; uterine cervix cancer
Subjects: QS آناتومی انسان
QTفیزیولوژی
میکروب شناسی وایمنی شناسی QW
مقالات نمایه شده محققین دانشگاه در سایت ,Web of Science ,Scopus
Divisions: معاونت تحقیقات و فناوری
Depositing User: GOUMS
Date Deposited: 30 Jun 2019 05:56
Last Modified: 30 Jun 2019 05:56
URI: http://eprints.goums.ac.ir/id/eprint/10122

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